{
  "$schema": "http://json-schema.org/draft-07/schema#",
  "$id": "https://schema.humancellatlas.org/module/biomaterial/6.2.1/growth_conditions",
  "description": "Information relating to how a biomaterial was grown and/or maintained in a laboratory setting.",
  "additionalProperties": false,
  "title": "growth_conditions",
  "type": "object",
  "properties": {
    "describedBy": {
      "description": "The URL reference to the schema.",
      "type": "string",
      "pattern": "^(http|https)://schema.(.*?)humancellatlas.org/module/biomaterial/(([0-9]{1,}.[0-9]{1,}.[0-9]{1,})|([a-zA-Z]*?))/growth_conditions"
    },
    "schema_version": {
      "description": "The version number of the schema in major.minor.patch format.",
      "type": "string",
      "pattern": "^[0-9]{1,}.[0-9]{1,}.[0-9]{1,}$",
      "example": "4.6.1"
    },
    "passage_number": {
      "description": "The number of passages (as an integer) that the biomaterial has been through.",
      "maximum": 1000,
      "minimum": 0,
      "type": "integer",
      "example": 22,
      "user_friendly": "Passage number"
    },
    "growth_medium": {
      "description": "The solid, liquid, or semi-solid medium used to support growth.",
      "type": "string",
      "example": "lysogeny broth (LB) medium",
      "user_friendly": "Growth medium"
    },
    "mycoplasma_testing_method": {
      "description": "The method by which the biomaterial was tested for mycoplasma contamination.",
      "type": "string",
      "enum": [
        "Direct DNA stain",
        "Indirect DNA stain",
        "Broth and agar culture",
        "PCR",
        "Nested PCR",
        "ELISA",
        "Autoradiography",
        "Immunostaining",
        "Cell-based assay",
        "Microbiological assay"
      ],
      "example": "Should be one of: Direct DNA stain, Indirect DNA stain, Broth and agar culture, PCR, Nested PCR, ELISA, Autoradiography, Immunostaining, Cell-based assay, or Microbiological assay.",
      "user_friendly": "Mycoplasma testing method"
    },
    "mycoplasma_testing_results": {
      "description": "Whether the biomaterial passed or failed the mycoplasma test.",
      "type": "string",
      "enum": [
        "pass",
        "fail"
      ],
      "example": "Should be one of: pass, or fail.",
      "user_friendly": "Mycoplasma testing results"
    },
    "drug_treatment": {
      "description": "Description of drugs added to the growth medium.",
      "type": "string",
      "example": "100 ug/mL ampicillin",
      "user_friendly": "Drug treatment"
    },
    "feeder_layer_type": {
      "description": "Type of feeder layer cells biomaterial was grown on.",
      "type": "string",
      "enum": [
        "feeder-free",
        "feeder-dependent, JK1 feeder cells",
        "feeder-dependent, SNL 76/7 feeder cells",
        "feeder-dependent, human marrow stromal cells",
        "feeder-dependent, bovine embryonic fibroblast cells",
        "feeder-dependent, mouse embryonic fibroblast cells",
        "feeder-dependent, mouse fibroblast STO cells",
        "feeder-dependent, mouse bone marrow stromal cells",
        "feeder-dependent, mouse yolk sac-derived endothelial cells",
        "feeder-dependent, human foreskin fibroblast cells",
        "feeder-dependent, human newborn fibroblast cells",
        "feeder-dependent, human fetal lung fibroblast cells",
        "feeder-dependent, human uterine endometrial cells",
        "feeder-dependent, human breast parenchymal cells",
        "feeder-dependent, human embryonic fibroblast cells",
        "feeder-dependent, human adipose stromal cells",
        "feeder-dependent, human amniotic epithelial cells",
        "feeder-dependent, human placental fibroblast cells",
        "feeder-dependent, human umbilical cord stromal cells",
        "feeder-dependent, human fetal muscle cells",
        "feeder-dependent, human fetal skin cells",
        "feeder-dependent, human fetal liver stromal cells",
        "feeder-dependent, human fallopian tubal epithelial cells",
        "feeder-dependent, human amniotic mesenchymal cells"
      ],
      "example": "feeder-dependent, mouse embryonic fibroblast cells",
      "user_friendly": "Feeder layer type"
    }
  }
}