{
  "$schema": "http://json-schema.org/draft-04/schema#",
  "$id": "https://schema.humancellatlas.org/module/protocol/0.0.0/targets",
  "description": "Information about a single microscope channel.",
  "additionalProperties": false,
  "required": [
    "assay_type",
    "multiplexed",
    "channel"
  ],
  "title": "Imaging target",
  "name": "imaging_target",
  "type": "object",
  "properties": {
    "describedBy": {
      "description": "The URL reference to the schema.",
      "type": "string",
      "pattern": "^(http|https)://schema.(.*?)humancellatlas.org/module/process/(([0-9]{1,}.[0-9]{1,}.[0-9]{1,})|([a-zA-Z]*?))/imaging_channels"
    },
    "schema_version": {
      "description": "The version number of the schema in major.minor.patch format.",
      "type": "string",
      "pattern": "^[0-9]{1,}.[0-9]{1,}.[0-9]{1,}$",
      "example": "4.6.1"
    },
    "target_molecule_name": {
      "description": "The name of a target molecule (small molecule or gene product) whose distribution is assayed by this experiment. If there is an accompanying code book, the name here should correspond to the target name used in the code book.",
      "type": "string",
      "example": "ACTA1",
      "user_friendly": "Target molecule name"
    },
    "target_molecule_ID": {
      "description": "An identifier referring to the the target molecule. For small molecules this should be from the ChEBI ontology. For gene products this should be a standard gene or gene product identifier (e.g. ensembl, uniprot).",
      "type": "string",
      "example": "CHEBI:85345, ensembl_9606",
      "user_friendly": "Target molecule identifier."
    },
    "target_subcellular_structure": {
      "description": "Target subcellular structure. This should be a term from the GO cell component ontology.",
      "type": "object",
      "$ref": "https://schema.humancellatlas.org/module/ontology/0.0.0/cellular_component_ontology",
      "user_friendly": "Target subcellular structure"
    },
    "reagent_name": {
      "description": "Name of reagent used to detect target.",
      "type": "string",
      "user_friendly": "Reagent name"
    },
    "purchased_reagent_details": {
      "description": "Name of reagent used to detect target.",
      "type": "object",
      "$ref": "https://schema.humancellatlas.org/module/process/6.0.2/purchased_reagents",
      "user_friendly": "Purchased reagent details"
    },
    "probe_sequence": {
      "pattern": "[ATGCUatgcu]+",
      "description": "Sequence of a probe used to capture the target.",
      "type": "string",
      "user_friendly": "Probe sequence"
    },
    "fluorophore": {
      "description": "Fluorophore used to detect target in non-multiplexed experiments.",
      "type": "string",
      "example": "FITC",
      "user_friendly": "Fluorophore"
    },
    "assay_type": {
      "description": "Type of assay used to detect target e.g. MERFISH",
      "type": "object",
      "$ref": "https://schema.humancellatlas.org/module/ontology/5.3.1/process_type_ontology",
      "example": "MerFish, smFISH, immunofluorescence, fluorescent cell stain",
      "user_friendly": "Assay type"
    },
    "multiplexed": {
      "description": "Is this target part of a multiplexed experiment? Should be yes or no.",
      "type": "string",
      "enum": [
        "yes",
        "no"
      ],
      "example": "yes",
      "user_friendly": "Multiplexed?"
    },
    "channel": {
      "description": "One or more channel names corresponding channels in imaging channels tab.",
      "type": "string",
      "example": "far red",
      "user_friendly": "Channel"
    }
  }
}