{
  "$schema": "http://json-schema.org/draft-07/schema#",
  "$id": "https://schema.humancellatlas.org/module/protocol/1.0.7/target",
  "description": "Information about a single microscope channel.",
  "additionalProperties": false,
  "required": [
    "assay_type",
    "multiplexed"
  ],
  "title": "Target",
  "name": "target",
  "type": "object",
  "properties": {
    "describedBy": {
      "description": "The URL reference to the schema.",
      "type": "string",
      "pattern": "^(http|https)://schema.(.*?)humancellatlas.org/module/protocol/(([0-9]{1,}.[0-9]{1,}.[0-9]{1,})|([a-zA-Z]*?))/target"
    },
    "schema_version": {
      "description": "The version number of the schema in major.minor.patch format.",
      "type": "string",
      "pattern": "^[0-9]{1,}.[0-9]{1,}.[0-9]{1,}$",
      "example": "4.6.1"
    },
    "molecule_name": {
      "description": "The name of a target molecule (small molecule or gene product) whose distribution is assayed by this experiment.",
      "type": "string",
      "example": "ACTA1; cFos",
      "user_friendly": "Target molecule name",
      "guidelines": "If there is an accompanying codebook, this name should correspond to the target name used in the codebook."
    },
    "molecule_id": {
      "description": "An identifier referring to the the target molecule.",
      "type": "string",
      "example": "CHEBI:85345; ensembl_9606",
      "user_friendly": "Target molecule identifier",
      "guidelines": "For small molecules this should be from the ChEBI ontology. For gene products this should be a standard gene or gene product identifier (e.g. ensembl, uniprot)."
    },
    "subcellular_structure": {
      "description": "Target subcellular structure. This should be a term from the GO cell component ontology.",
      "type": "object",
      "$ref": "https://schema.humancellatlas.org/module/ontology/1.0.4/cellular_component_ontology",
      "user_friendly": "Target subcellular structure"
    },
    "reagent_name": {
      "description": "Name of reagent used to detect target.",
      "type": "string",
      "user_friendly": "Reagent name",
      "guidelines": "Please list any encoding probes, readout probes, oligonucleotide probes conjugated to secondary antibody."
    },
    "purchased_reagent_details": {
      "description": "Information describing purchased reagent used to detect target.",
      "type": "object",
      "$ref": "https://schema.humancellatlas.org/module/process/6.0.4/purchased_reagents",
      "user_friendly": "Purchased reagent details"
    },
    "probe_sequence": {
      "pattern": "[ATGCUatgcu]+",
      "description": "Sequence of a probe used to detect target.",
      "type": "string",
      "user_friendly": "Probe sequence"
    },
    "fluorophore": {
      "description": "Fluorophore used to detect target.",
      "type": "string",
      "example": "FITC",
      "user_friendly": "Fluorophore"
    },
    "assay_type": {
      "description": "Type of assay used to detect target.",
      "type": "object",
      "$ref": "https://schema.humancellatlas.org/module/ontology/5.3.4/process_type_ontology",
      "example": "MERFISH; smFISH; immunofluorescence; fluorescent cell stain",
      "user_friendly": "Assay type"
    },
    "multiplexed": {
      "description": "Were multiple targets detected simultaneously in one channel? Should be yes or no.",
      "type": "string",
      "enum": [
        "yes",
        "no"
      ],
      "example": "yes",
      "user_friendly": "Is this a multiplexed experiment?"
    },
    "channel_id": {
      "description": "Channel ID used to assay signal. Should be consistent with the ID in the channel tab.",
      "type": "array",
      "items": {
        "type": "string"
      },
      "example": "1; A",
      "user_friendly": "Channel"
    }
  }
}