{
  "$schema": "http://json-schema.org/draft-07/schema#",
  "$id": "https://schema.humancellatlas.org/type/protocol/biomaterial_collection/0.0.0/treatment_protocol",
  "description": "Information about how cells are subjected to a treatment or stimulus, such as an immune activator or a drug.",
  "additionalProperties": false,
  "required": [
    "describedBy",
    "schema_type",
    "protocol_core",
    "method"
  ],
  "title": "Treatment protocol",
  "name": "treatment_protocol",
  "type": "object",
  "properties": {
    "describedBy": {
      "description": "The URL reference to the schema.",
      "type": "string",
      "pattern": "^(http|https)://schema.(.*?)humancellatlas.org/type/protocol/biomaterial_collection/(([0-9]{1,}.[0-9]{1,}.[0-9]{1,})|([a-zA-Z]*?))/treatment_protocol"
    },
    "schema_version": {
      "description": "The version number of the schema in major.minor.patch format.",
      "type": "string",
      "pattern": "^[0-9]{1,}.[0-9]{1,}.[0-9]{1,}$",
      "example": "4.6.1"
    },
    "schema_type": {
      "description": "The type of the metadata schema entity.",
      "type": "string",
      "enum": [
        "protocol"
      ]
    },
    "provenance": {
      "description": "Provenance information provided by the system.",
      "type": "object",
      "$ref": "https://schema.humancellatlas.org/system/1.1.0/provenance"
    },
    "protocol_core": {
      "description": "Core protocol-level information.",
      "type": "object",
      "$ref": "https://schema.humancellatlas.org/core/protocol/5.2.5/protocol_core",
      "user_friendly": "Protocol core"
    },
    "method": {
      "description": "Method applied to cell culture to induce a specific treatment response.",
      "type": "string",
      "user_friendly": "Treatment method",
      "comment": "Consider making this an ontology in the future.",
      "example": "Embryoid Body; Monolayer; Inductive Co-Culture"
    },
    "treatment": {
      "description": "A list of treatment types used to treat a biomaterial.",
      "type": "string",
      "user_friendly": "Treatment",
      "example": "Human CD3/CD28 T Cell Activator; TNF; proGLC"
    },
    "treatment_effect": {
      "description": "A biological process that a treatment is intended to activate or modify.",
      "type": "string",
      "user_friendly": "Treatment effect",
      "example": "TCR stimulation; immune cell activation; signalling pathway inhibition"
    },
    "media": {
      "description": "Culture media used to induce a specific treatment response.",
      "type": "string",
      "user_friendly": "Treatment media",
      "example": "RPMI 1640; Complete Medium"
    },
    "reagents": {
      "description": "A list of purchased reagents used in the treatment protocol.",
      "type": "array",
      "items": {
        "type": "object",
        "$ref": "https://schema.humancellatlas.org/module/process/6.1.0/purchased_reagents"
      },
      "user_friendly": "Treatment reagents"
    },
    "target_pathway": {
      "description": "Targeted pathway for specific treatment response.",
      "type": "string",
      "user_friendly": "Target pathway",
      "example": "insulin signalling pathway; immune cell activation; chemokine signalling pathway."
    }
  }
}