{
  "$schema": "http://json-schema.org/draft-07/schema#",
  "$id": "https://schema.humancellatlas.org/type/protocol/biomaterial_collection/2.0.4/ipsc_induction_protocol",
  "description": "Information about how a biomaterial is treated to become an induced pluripotent stem cell.",
  "additionalProperties": false,
  "required": [
    "describedBy",
    "schema_type",
    "protocol_core",
    "ipsc_induction_method"
  ],
  "title": "iPSC induction protocol",
  "name": "ipsc_induction_protocol",
  "type": "object",
  "properties": {
    "describedBy": {
      "description": "The URL reference to the schema.",
      "type": "string",
      "pattern": "^(http|https)://schema.(.*?)humancellatlas.org/type/protocol/biomaterial_collection/(([0-9]{1,}.[0-9]{1,}.[0-9]{1,})|([a-zA-Z]*?))/ipsc_induction_protocol"
    },
    "schema_version": {
      "description": "The version number of the schema in major.minor.patch format.",
      "type": "string",
      "pattern": "^[0-9]{1,}.[0-9]{1,}.[0-9]{1,}$",
      "example": "4.6.1"
    },
    "schema_type": {
      "description": "The type of the metadata schema entity.",
      "type": "string",
      "enum": [
        "protocol"
      ]
    },
    "provenance": {
      "description": "Provenance information provided by the system.",
      "type": "object",
      "$ref": "https://schema.humancellatlas.org/system/1.0.3/provenance"
    },
    "protocol_core": {
      "description": "Core protocol-level information.",
      "type": "object",
      "$ref": "https://schema.humancellatlas.org/core/protocol/5.2.5/protocol_core",
      "user_friendly": "Protocol core"
    },
    "ipsc_induction_method": {
      "description": "Induction method applied to primary cell culture to induce pluripotent stem cell generation.",
      "type": "string",
      "enum": [
        "lentivirus",
        "sendai virus",
        "Gun particle",
        "piggyBac transposon",
        "miRNA viral",
        "adenovirus",
        "cre-loxP",
        "plasmid",
        "retroviral"
      ],
      "user_friendly": "Induction method",
      "comment": "Consider making this field an ontology in the future",
      "example": "Should be one of: lentivirus, sendai virus, Gun particle, piggyBac transposon, miRNA viral, adenovirus, cre-loxP, plasmid, or retroviral."
    },
    "ipsc_induction_factors": {
      "description": "Induction factors added to primary cell culture to induce pluripotency.",
      "type": "string",
      "user_friendly": "Induction factors",
      "example": "POU5F1; SOX2; KLF4; c-MYC"
    },
    "ipsc_induction_kit": {
      "description": "Kit used to induce pluripotent stem cell generation.",
      "type": "object",
      "$ref": "https://schema.humancellatlas.org/module/process/6.0.4/purchased_reagents",
      "user_friendly": "Induction kit"
    },
    "pluripotency_test": {
      "description": "Description of how pluripotency was tested in induced pluripotent stem cells.",
      "type": "string",
      "user_friendly": "Pluripotency test",
      "example": "Teratoma formation; PluriTest; Rt-PCR"
    },
    "percent_pluripotency": {
      "description": "Percent of iPSCs that passed the pluripotency test.",
      "type": "number",
      "example": 97.2,
      "user_friendly": "Percent pluripotency"
    },
    "pluripotency_vector_removed": {
      "description": "Whether a viral vector was removed after induction.",
      "type": "string",
      "enum": [
        "yes",
        "no",
        "unknown"
      ],
      "user_friendly": "Pluripotent vector removed?",
      "example": "Should be one of: yes, no, or unknown."
    },
    "ipsc_induction_produced_in_house": {
      "description": "Whether the induced pluripotent stem cell was prepared in-house.",
      "type": "boolean",
      "user_friendly": "iPSC prepared in-house?",
      "example": "Should be one of: yes, or no."
    },
    "protocol_reagents": {
      "description": "A list of additional purchased reagents used in this protocol.",
      "type": "array",
      "items": {
        "type": "object",
        "$ref": "https://schema.humancellatlas.org/module/process/6.0.4/purchased_reagents"
      },
      "user_friendly": "Additional protocol reagents"
    }
  }
}