{
  "$schema": "http://json-schema.org/draft-07/schema#",
  "$id": "https://schema.humancellatlas.org/type/protocol/biomaterial_collection/2.2.0/differentiation_protocol",
  "description": "Information about how a cell is differentiated to a desired cell type or organoid.",
  "additionalProperties": false,
  "required": [
    "describedBy",
    "schema_type",
    "protocol_core",
    "method"
  ],
  "title": "Differentiation protocol",
  "name": "differentiation_protocol",
  "type": "object",
  "properties": {
    "describedBy": {
      "description": "The URL reference to the schema.",
      "type": "string",
      "pattern": "^(http|https)://schema.(.*?)humancellatlas.org/type/protocol/biomaterial_collection/(([0-9]{1,}.[0-9]{1,}.[0-9]{1,})|([a-zA-Z]*?))/differentiation_protocol"
    },
    "schema_version": {
      "description": "The version number of the schema in major.minor.patch format.",
      "type": "string",
      "pattern": "^[0-9]{1,}.[0-9]{1,}.[0-9]{1,}$",
      "example": "4.6.1"
    },
    "schema_type": {
      "description": "The type of the metadata schema entity.",
      "type": "string",
      "enum": [
        "protocol"
      ]
    },
    "provenance": {
      "description": "Provenance information provided by the system.",
      "type": "object",
      "$ref": "https://schema.humancellatlas.org/system/1.1.0/provenance"
    },
    "protocol_core": {
      "description": "Core protocol-level information.",
      "type": "object",
      "$ref": "https://schema.humancellatlas.org/core/protocol/5.2.5/protocol_core",
      "user_friendly": "Protocol core"
    },
    "method": {
      "description": "Method applied to cell culture to induce a specific differentiation response.",
      "type": "string",
      "user_friendly": "Differentiation method",
      "comment": "Consider making this an ontology in the future.",
      "example": "Embryoid Body; Monolayer; Inductive Co-Culture"
    },
    "media": {
      "description": "Culture media used to induce a specific differentiation response.",
      "type": "string",
      "user_friendly": "Differentiation media",
      "example": "RPMI 1640 + B27; Neurobasal Media; StemPro-34 Serum-Free Medium"
    },
    "small_molecules": {
      "description": "Small molecules added to stem cell medium to induce a specific differentiation response.",
      "type": "string",
      "user_friendly": "Small molecules",
      "example": "Retinoic Acid; CHIR99021 (GSK-inhibitor); Activin A; BMP4"
    },
    "target_cell_yield": {
      "description": "Percent of target cells obtained after directed differentiation of origin cell.",
      "type": "number",
      "example": 95,
      "user_friendly": "Percent target cell yield"
    },
    "reagents": {
      "description": "A list of purchased reagents used in the differentiation protocol.",
      "type": "array",
      "items": {
        "type": "object",
        "$ref": "https://schema.humancellatlas.org/module/process/6.1.0/purchased_reagents"
      },
      "user_friendly": "Differentiation reagents"
    },
    "target_pathway": {
      "description": "Targeted pathway for specific differentiation response.",
      "type": "string",
      "user_friendly": "Target pathway",
      "example": "Wnt pathway; Hedgehog signaling pathway",
      "comment": "Consider making this an ontology in the future."
    },
    "validation_method": {
      "description": "Method used to validate origin cell successfully differentiated to target cell.",
      "type": "string",
      "user_friendly": "Differentiation validation method",
      "example": "Pancreatic Cell DTZ Detection Assay; qPCR; Flow Cytometry; Immunocytochemistry Staining"
    },
    "validation_result": {
      "description": "Result confirming successful differentiation to target cell type.",
      "type": "string",
      "user_friendly": "Validation result",
      "example": "CD103 Positive; Nestin Positive; HCN4 Positive; CD11C Negative"
    }
  }
}