{
  "$schema": "http://json-schema.org/draft-07/schema#",
  "$id": "https://schema.humancellatlas.org/type/protocol/biomaterial_collection/3.0.0/enrichment_protocol",
  "description": "Information about how a biomaterial is enriched for a feature or characteristic of interest.",
  "additionalProperties": false,
  "required": [
    "describedBy",
    "schema_type",
    "protocol_core",
    "method"
  ],
  "title": "Enrichment protocol",
  "name": "enrichment_protocol",
  "type": "object",
  "properties": {
    "describedBy": {
      "description": "The URL reference to the schema.",
      "type": "string",
      "pattern": "^(http|https)://schema.(.*?)humancellatlas.org/type/protocol/biomaterial_collection/(([0-9]{1,}.[0-9]{1,}.[0-9]{1,})|([a-zA-Z]*?))/enrichment_protocol"
    },
    "schema_version": {
      "description": "The version number of the schema in major.minor.patch format.",
      "type": "string",
      "pattern": "^[0-9]{1,}.[0-9]{1,}.[0-9]{1,}$",
      "example": "4.6.1"
    },
    "schema_type": {
      "description": "The type of the metadata schema entity.",
      "type": "string",
      "enum": [
        "protocol"
      ]
    },
    "provenance": {
      "description": "Provenance information provided by the system.",
      "type": "object",
      "$ref": "https://schema.humancellatlas.org/system/1.0.3/provenance"
    },
    "protocol_core": {
      "description": "Core protocol-level information.",
      "type": "object",
      "$ref": "https://schema.humancellatlas.org/core/protocol/5.2.5/protocol_core",
      "user_friendly": "Protocol core"
    },
    "method": {
      "description": "The method by which enrichment was achieved.",
      "type": "object",
      "$ref": "https://schema.humancellatlas.org/module/ontology/5.3.4/process_type_ontology",
      "user_friendly": "Enrichment method"
    },
    "markers": {
      "description": "A list of markers used to enrich for or against certain cells.",
      "type": "string",
      "example": "CD4+ CD8-; HLA-G+",
      "user_friendly": "Markers",
      "guidelines": "Format as a space-delimited list of markers with +/- where + means enriched for and - means enriched against."
    },
    "minimum_size": {
      "description": "Minimum cell or organelle size passing selection, in microns.",
      "type": "number",
      "maximum": 10000,
      "minimum": 0.01,
      "user_friendly": "Minimum size selected",
      "example": 70
    },
    "maximum_size": {
      "description": "Maximum cell or organelle size passing selection, in microns.",
      "type": "number",
      "maximum": 10000,
      "minimum": 0.01,
      "user_friendly": "Maximum size selected",
      "example": 90
    }
  }
}