{
  "$schema": "http://json-schema.org/draft-07/schema#",
  "$id": "https://schema.humancellatlas.org/type/protocol/imaging/2.2.0/imaging_preparation_protocol",
  "description": "Information about the preparation protocol of the imaged specimen used in an imaging experiment.",
  "additionalProperties": false,
  "required": [
    "describedBy",
    "schema_type",
    "protocol_core"
  ],
  "title": "Imaging preparation protocol",
  "name": "imaging_preparation_protocol",
  "type": "object",
  "properties": {
    "describedBy": {
      "description": "The URL reference to the schema.",
      "type": "string",
      "pattern": "^(http|https)://schema.(.*?)humancellatlas.org/type/protocol/imaging/(([0-9]{1,}.[0-9]{1,}.[0-9]{1,})|([a-zA-Z]*?))/imaging_preparation_protocol"
    },
    "schema_version": {
      "description": "The version number of the schema in major.minor.patch format.",
      "type": "string",
      "pattern": "^[0-9]{1,}.[0-9]{1,}.[0-9]{1,}$",
      "example": "4.6.1"
    },
    "schema_type": {
      "description": "The type of the metadata schema entity.",
      "type": "string",
      "enum": [
        "protocol"
      ]
    },
    "provenance": {
      "description": "Provenance information provided by the system.",
      "type": "object",
      "$ref": "https://schema.humancellatlas.org/system/1.1.0/provenance"
    },
    "protocol_core": {
      "description": "Core protocol-level information.",
      "type": "object",
      "$ref": "https://schema.humancellatlas.org/core/protocol/5.2.5/protocol_core",
      "user_friendly": "Protocol core"
    },
    "fresh_slicing_method": {
      "description": "The method by which fresh tissue was sliced.",
      "type": "string",
      "user_friendly": "Fresh slicing method",
      "example": "vibrotome"
    },
    "imaged_slice_thickness": {
      "description": "Thickness of the imaged slice in micrometres.",
      "type": "number",
      "example": "10",
      "user_friendly": "Imaged slice thickness"
    },
    "final_slicing_method": {
      "description": "The method by which the final slice was obtained.",
      "type": "string",
      "user_friendly": "Final slicing method",
      "example": "cryosectioning"
    },
    "post_resection_interval": {
      "description": "Length of time between surgical resection and fresh slicing of tissue.",
      "type": "number",
      "user_friendly": "Time between resection and fresh slicing",
      "example": "5"
    },
    "post_resection_interval_unit": {
      "description": "The unit of time in which the post resection interval is expressed.",
      "type": "object",
      "$ref": "https://schema.humancellatlas.org/module/ontology/5.3.5/time_unit_ontology",
      "user_friendly": "Post resection interval time unit",
      "example": "day"
    },
    "pre_final_slice_preservation_method": {
      "description": "Tissue preservation method used prior to final slicing.",
      "type": "object",
      "$ref": "https://schema.humancellatlas.org/module/biomaterial/6.1.1/preservation_storage",
      "user_friendly": "Pre-final slice preservation method",
      "example": "freezing"
    },
    "post_final_slicing_interval": {
      "description": "Length of time between secondary slicing and hybridization.",
      "type": "number",
      "example": "7",
      "user_friendly": "Post final slicing interval"
    },
    "post_final_slicing_interval_unit": {
      "description": "The unit of time in which the post final slicing interval is expressed.",
      "type": "object",
      "$ref": "https://schema.humancellatlas.org/module/ontology/5.3.5/time_unit_ontology",
      "user_friendly": "Post final slicing interval time unit",
      "example": "day"
    },
    "fiducial_marker": {
      "description": "Fiducial markers for the alignment of images taken across multiple rounds of imaging.",
      "type": "string",
      "user_friendly": "Fiducial marker",
      "example": "beads"
    },
    "expansion_factor": {
      "description": "Factor by which the imaged tissue was expanded in one dimension.",
      "type": "number",
      "example": "3",
      "user_friendly": "Expansion factor"
    }
  }
}